焦磷酸测序法分析中国荷斯坦牛、娟姗牛及水牛的乳蛋白基因多态性

Genetic polymorphisms of milk protein in Chinese Holstein cattle, Jersey cattle and water buffalo analyzed by pyrosequencing

目的本研究旨在分析和比较中国荷斯坦牛、娟姗牛和水牛的乳蛋白基因多态性。方法根据奶牛多态位点设计引物,采用焦磷酸测序法分析乳蛋白基因多态性,并采用高效液相色谱法进行验证。结果荷斯坦牛和娟姗牛的乳蛋白(β-casein,κ-casein和β-lactoglobulin)均存在基因多态性,而水牛仅在κ-casein上存在多态性且多态位点与另两种奶牛不同。结论中国荷斯坦牛、娟姗牛和水牛的乳蛋白基因均存在多态性,焦磷酸测序法能高通量、快速测定乳蛋白基因多态性。

Objective To analyze and compare the genetic polymorphisms of milk protein in Chinese Holstein cattle, Jersey cattle and water buffalo. Methods The primers were designed according to the milk protein polymorphic sites of Holstein, and the genetic polymorphisms were analyzed by pyrosequencing and confirmed by RP-HPLC. Results Genetic polymorphisms of milk protein genes(β-casein, κ-casein and β-lactoglobulin) were detected in Chinese Holstein cattle and Jersey cattle. However, no polymorphism was found in water buffalo except κ-casein, and the polymorphic site was different with Holstein and Jersey. Conclusion Milk protein polymorphism was found in Chinese Holstein, Jersey and buffalo, and pyrosequencing was a high-throughput and fast method for milk protein genotyping.