超高效液相色谱-串联质谱法测定鸡组织和牛组织中的甲苄喹啉和癸氧喹酯

Determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry

目的建立超高效液相色谱-串联质谱检测鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测方法。方法样品采用乙腈提取,亲水-亲脂平衡介质固相萃取柱(hydrophile-lipophile balance,HLB)净化,超高效液相色谱-串联质谱仪检测,外标法定量。结果甲苄喹啉和癸氧喹酯在0.005~0.250 mg/kg范围内线性关系良好,相关系数均大于0.999,方法定量限为0.01 mg/kg,平均回收率范围为77.0%~110%,相对标准偏差范围为1.36%~12.4%。结论 本方法简便、快速、准确、灵敏度高,适用于鸡组织和牛组织中甲苄喹啉和癸氧喹酯残留的检测与确证。

Objective Todevelop a method for determination of nequinate and decoquinate residues in chicken tissues and bovine tissues by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The residues of nequinate and decoquinate in the test sample were extracted with acetonitrile. The extraction was cleaned with hydrophile-lipophile balance (HLB) cartridge. The residues were determined by UPLC-MS/MS, using external standard method.Results The limits of quantification were 0.010 mg/kg for nequinate and decoquinate, the standard curves were linear in the range of 0.005~0.250 mg/kg, with the correlation coefficients >0.999. The average recovery was between 77.0%~110%, the RSD was be-tween 1.36%~12.4%.Conclusion This method is rapid, simple, precise, and sensitive for both confirmation and quantification of nequinate and decoquinate residues in chicken tissues and bovine tissues.