超高效液相色谱法快速检测麦卢卡蜂蜜中丙酮醛

Determination of methylglyoxal in Manuka honey by ultra performance liquid chromatography

目的利用超高效液相色谱建立一种麦卢卡蜂蜜中丙酮醛的快速检测方法。方法采用乙酸钠缓冲溶液直接提取蜂蜜中的丙酮醛,提取液与2,4-二硝基苯肼溶液室温避光衍生20 h后,经C18色谱柱分离后采用二极管阵列检测器(PDA)检测,外标法定量。结果丙酮醛的检出限为10.0 mg/kg,定量限为25.0 mg/kg,在1.0~60 mg/L之间线性关系良好,相关系数为0.9982,在蜂蜜中添加25.0、500、1000三个水平的回收率在93.9%~97.8%之间,相对标准偏差在1.8%~5.4%之间。结论 该分析方能够满足麦卢卡蜂蜜中丙酮醛的快速测定要求。

Objective To establish a fast detection method of methylglyoxal (MGO) in Manuka honey by ultra performance liquid chromatography (UPLC).MethodsMGO in honey was extracted by sodium acetate buffer solution, extract solution derivatized 20 h with 2,4-dinitrophenylhydrazine solution, and detected by PDA detector after separated by C18 chromatographic column, quantified by external standard calibration me-thod.Results The detection limit of MGO was 10.0 mg/kg, limit of quantification was 25.0 mg/kg. The me-thod showed a good linear range of detection between 1.0~60 mg/L mg/L (r2=0.9982). The recoveries of MGO at three spiked levels (25.0, 500, and 1000 mg/kg) were between 93.9% ~ 97.8 %, RSD between 1.8% ~ 5.4%. Conclusion The method is suitable for fast analysis of MGO in Manuka honey.