光纤倏逝波生物传感器在食品安全检测中应用进展

Progress on fiber-optic evanescent wave biosensor technique in food safety detection

光纤倏逝波生物传感主要是利用倏逝波场来激发光纤表面标记在生物分子(抗体或核酸片段)上的荧光染料,从而检测通过特异性反应附着于纤芯表面倏逝波场范围内的生物分子的一项新兴免疫检测技术,具有灵敏度高、特异性强、使用便捷、检测速度快等特点,已被应用于食品安全快速检测过程中。本文对光纤倏逝波生物传感技术在食品安全检测领域的应用进行了综述,介绍了光纤倏逝波生物传感器的原理,通过分析光纤倏逝波生物传感器目前在食品检测项目的具体应用,主要包括:在食品微生物及其毒素检测中的应用,在食品理化检测中的应用,对其特点进行了讨论。我国在该领域的工作尚处于研发阶段,还有大量的工作需要开展,一旦获得成效势必给我国食品安全问题提供有力的保障,为我国食品工业发展提供重要支撑。

Fiber-optic evanescent wave biosensor is a new immunoassay technology, with the use of exploiting the evanescent wave field to excite the fluorescent dye marked on the biological molecule (antibody or nucleic acid fragments) on the surface of fiber surface. It can detect the biological molecules adhering to the surface of fiber core within the scope of evanescent wave by specific immune reaction. With the outstanding characteristics such as high sensitivity, specificity, simplicity and high determination speed, the technology is widely used in food safety rapid detection. This study reviewed the application of fiber-optic evanescent wave biosensor in the field of food safety detection, by analyzing the specific application of food detection project in current, which mainly included: the detection application in food microorganism and toxin, the detection application in food physical and chemical, and the characteristics were discussed. Our work in this area was still in the developing stage, there were a lot of work to do, and once success would provide strong safeguard effects to China's food safety problem and important support for China's food industry development.