气相色谱质谱-内标法测定植物油中9种农药的残留量

Determination of 9 pesticide residues in vegetable oil by gas chromatographymass spectrometry inner standard method

目的建立植物油中9种农药残留量的内标检测方法。方法样品用乙腈提取,经优化的Qu ECh ERS方法净化,气相色谱-质谱联用仪测定,内标法定量。结果氟乐灵、甲拌磷、七氯、倍硫磷、反-氯丹、顺-氯丹的线性范围为0.005~0.2 mg/L,检出限为0.01 mg/kg;α-硫丹、β-硫丹的线性范围为0.01~0.2 mg/L,检出限为0.02 mg/kg;多效唑的线性范围为0.02~0.2 mg/L,检出限为0.04 mg/kg。结论本方法准确度高、重复性好,前处理无需特殊仪器,且内标物的引入进一步降低了前处理的操作难度,为方法的推广创造了更好的条件。

Objective To establish an inner standard method for the simultaneous determination of 9 pesticide residues in vegetable oil. Methods The samples were extracted by acetonitrile and then purified by QuEChERS method, the supernatant was analyzed by gas chromatography-mass spectrometry using an inner standard method. Results The linear range and LOD of were 0.005~0.2 mg/L and 0.01 mg/kg for trifluralin, phorate, heptachlor, fenthion, α-chlordane,β-chlordane, and 0.01~0.2 mg/L and 0.02 mg/kg for α-endosulfan,β-endosulfan, and 0.02~0.2 mg/L and 0.04 mg/kg for paclobutrazol. Conclusion The method is simple, reliable and does not need special equipments during the sample pre-treatment.