吡唑醚菌酯残留分析研究进展

Development of methods for the determination of pyraclostrobin residue

吡唑醚菌酯是一种新型甲氧基丙烯酸酯类杀菌剂,广泛用于防治水果、蔬菜、谷物等作物中的白粉病、锈病、网斑病、霜霉病、稻瘟病等病害。本文对吡唑醚菌酯的残留分析方法进行了综述。在样品前处理中,以octadecyl silica(C18)、N-丙基乙二胺或石墨化碳黑为填料的固相萃取技术应用最为广泛;涂装聚二甲基硅氧烷、二乙烯基苯等的固相微萃取技术,由于具有很高的灵敏度近年来也已大量应用;基质固相分散、凝胶渗透色谱、分散固相萃取等技术的应用,使净化效率大大提高。在样品分析中,传统的气相色谱、高效液相色谱特别是后者虽然仍在大量应用,但气相色谱-串联质谱、高效液相色谱-串联质谱技术由于具有极高的选择性和灵敏性,已得到迅速发展和广泛应用。酶联免疫吸附分析、胶束电动毛细管色谱、近红外光谱技术等分析检测技术的应用潜力很大。

Pyraclostrobin is a kind of new strobilurin fungicide. It is widely used on a variety of crops, including fruits, vegetables and cereals, to control fungal diseases such as powdery mildew, rust, web blotch, downy mildew, rice blast and so on. In this article, methods for the determination of pyraclostrobin residue were reviewed. Solid-phase extraction (SPE) with standard solid supports such as octadecyl-silica (C18), primary secondary amine (PSA) or graphitized carbon black (GCB) had been widely used in pre-treatment of samples. Recently, solid-phase microextraction (SPME) had also been for the analysis of pyraclostrobin traces using polydimethylsiloxane, divinylbenzene, polyacrylate or carboxen fibers, with excellent sensitivity. The applications of matrix solid-phase dispersion (MSPD), gel permeation chromatography (GPC) and dispersive solid phase extraction (DSPE) had been shown to improve clean-up efficiency greatly. Although traditional gas chromatography and high-performance liquid chromatography especially the latter were still widely used in analysis of samples, gas chromatography-tandem mass spectrometry (GC–MS/MS) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) had been developed rapidly and applied widely because of their excellent selectivity and sensitivity. Enzyme-linked immunosorbent assays (ELISA), micellar electrokinetic capillary chromatography (MEKC) and near-infrared spectroscopy (NIR) had great application potentialities.