超高效液相色谱-串联质谱法快速测定饮料中22种添加剂

Simultaneous determination of 22 additives in beverage by ultra performance liquid chromatography-tandem mass spectrometry

目的建立了超高效液相色谱-串联质谱法(ultra high-performance liquid chromatography-tandem mass spectrometry,UHPLC-MS/MS)同时测定饮料中22种食用添加剂的方法,包括6种防腐剂,5种甜味剂,5种抗氧化剂和6种着色剂。方法试样经乙腈/水溶液超声提取,离心,上清液用水稀释后液相色谱-串联质谱仪直接测定,外标法定量,整个过程可在0.5 h内完成。结果在5~320μg/L的线性范围内,各物质线性相关系数均大于0.995。加标回收率范围为63.3%~107.9%,方法检测限在0.05~0.4 mg/kg之间,相对标准偏差在7.0%~14.8%之间。结论将本方法用于实际样品分析,简便快速,灵敏度高,可以满足对饮料中多种食品添加剂的同时测定。

Objective To establish a method for determination of 22 food additives, including 6 preservatives, 5 sweeteners, 5 antioxidants and 6 colorants in beverage by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) simultaneously. Methods Samples were extracted by acetonitrile/water and the extract was centrifuged at 10000 r/min for 10 min. Then supernatant was diluted and injected directly into the mass spectrometer. The whole procedure could be completed within 0.5 h. Quantification was based on external calibration. Results The correlation coefficient was higher than 0.995 in the range of 5~320μg/L. The average recovery of 22 food additives in fruit juice was 63.3%~107.9%. The limit of quantitation was from 0.05 mg/kg to 0.4 mg/kg and the relative standard deviation was between 7.0% and 14.8%. Conclusion This quantitative method has many advantages including simple pretreatment, rapid determination and high sensitivity, which can be applied to the real determination.