茯砖茶水提物对高脂血症小鼠脂质代谢的影响及其抗氧化作用的研究

Study on influence of Fuzhuan brick tea aqueous extract on lipid metabolism in hyperlipidemia mice and its antioxidation

目的考察茯砖茶的降血脂及肝保护作用。方法用高脂饲料饲喂小鼠建立高脂血症动物模型,以不同剂量(50 mg/kg·BW、150 mg/kg·BW、300 mg/kg·BW)茯砖茶水提物给小鼠每天灌胃,连续4周后检测血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平。同时观察其对肝组织谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)、体重、肝体比及肝组织病理变化的影响。结果结果显示,与模型组相比,茯砖茶水提物可呈剂量依赖性地降低血清中TC、TG、LDL-C的水平,提高HDL-C的含量。茯砖茶水提物还可以显著降低小鼠体重、肝体比及肝组织中MDA含量,极显著提高肝组织中GSH-Px和SOD活性,并减轻了肝脏的脂肪变性程度。结论茯砖茶水提物有较明显地改善脂质代谢和抗氧化的作用。

Objective To study the function of Fuzhuan brick tea aqueous extract on regulating blood lipid and alleviating liver impairment in hyperlipidemia mice.Methods The mice model with hyperlipidemia was established by feeding a high-fat diet. Fuzhuan brick tea aqueous extracts (50 mg/kg·BW, 150 mg/kg·BW and 300 mg/kg·BW) were administered to rats daily for 4 wk, respectively. The effects of Fuzhuan brick tea on serum levels of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C) were detected. Meanwhile, the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and malondialdehyde (MDA) of liver tissue, body weight and liver-to-body weight ratio were examined. Sections of mice liver tissue were stained with hematoxylin-eosin (HE) and histologically analyzed.Results Compared with model group, Fuzhuan brick tea decreased the serum TC, TG and LDL-C levels, and increased the level of HDL-C in a dose-dependent manner. In addition, Fuzhuan brick tea significantly reduced the body weigh, liver-to-body weight ratio and the level of MDA, and increased the GSH-Px and SOD activity. HE staining showed that the fat particles in liver cytoplasm were remarkably reduced by Fuzhuan brick tea.Conclusion Fuzhuan brick tea has an obvious hypolipidemic effect and antioxidative activity in mice.