液相色谱-串联质谱法测定鳗鱼中甲苯咪唑含量的不确定度评定

Uncertainty analysis on determination of the content of mebendazole in eel by liquid chromatography-mass spectrometry/maass spectrometry

目的探讨液相色谱-串联质谱法(LC-MS/MS)测定鳗鱼中甲苯咪唑含量的不确定度评价方法。方法根据JJF1059.1-2012《测量不确定度的评定与表示》,参考《化学分析中不确定度的评定的评估指南》及《实用测量不确定度评定》等相关理论,对测定过程中甲苯咪唑含量、样品定容体积、样品称量质量、称量样品稀释、样品重复性等引入的不确定度分量进行评定,评定鳗鱼中甲苯咪唑残留量测定的不确定度。结果测定结果合成不确定度为1.6×10-4 mg/kg,扩展不确定度为3.2×10-4 mg/kg。结论建立的不确定度评定方法适用于LC-MS/MS法测定鳗鱼中甲苯咪唑残留量的不确定度评定。

Objective To investigate the method that uncertainty evaluation of the determination of mebendazole content in eel by liquid chromatography-mass spectrometry/mass spectrometry (LC/MS/MS). Methods Based on JJF 1059.1-2012 Evaluation and expression of uncertainty in measurement, Guidance on evaluating the uncertainty in chemical analysis and The practical measurement uncertainty evaluation and other related theory, the uncertainty component that content of mebendazole, constant volume, mass, and the diluent and repetition for the sample were evaluated in the process of determination. Then uncertainty measurement of mebendazole residues in eel was evaluated. Results The results showed that the combined uncertainty of mebendazole residues in eel was 1.6×10-4 mg/kg, the expanded uncertainty was 3.2×10-4 mg/kg. Conclusion This uncertainty evaluation method is suitable for determination of mebendazole residues in eel by LC-MS/MS.