液相色谱法测定保健食品中硫酸软骨素的含量

Determination of chondroitin sulfate in health food by high performance liquid chromatography

目的建立一种快速、简便测定保健食品中硫酸软骨素的液相色谱方法,并进行方法学研究。方法样品经过前处理,以乙腈和0.005 mol/L戊烷磺酸钠溶液为流动相,等度洗脱,经Diamonsil C18柱分离,于192 nm波长检测。结果用本方法进行检测,能很好地分离硫酸软骨素,在浓度0.008 mg/m L^0.04 mg/m L之间呈现良好的线性关系,方法的相关系数可达1.000,平均回收率在98.5%~100.0%之间,相对标准偏差为0.6%。结论采用该方法能测定保健食品中硫酸软骨素,耗时短、操作简便、准确、重现性好,分离效果明显,可在实验室推广。

Objective To investigate a quick and simple method for determination of chondroitin sulfate in health foods by high performance liquid chromatography (HPLC). Methods Samples were treated using 0.005 mol/L pentane solution of sodium-acetonitrile as mobile phase with isocratic elution, separated on diamonsil C18 column, and detected at 192 nm wavelength. Results Chondroitin sulfate could be separated well by this method. It had a good linear relationship within 0.008~0.04 mg/mL (r=1.000), the average recoveries was 98.5%~100.0%, and the RSD was 0.6%. Conclusion The method can be used for determination of chondroitin sulfate in health foods, it is quick, simple and accurate, and has a good reproducibility and clear separation, which can be popularized in the laboratory.