食品中沙门氏菌检出能力验证结果与分析

Proficiency testing results and analysis of Salmonella detection ability in food

目的为了提升实验室的食品中沙门氏菌检测能力,增强实验室竞争能力,本实验室参加了中国食品药品检定研究院组织的NIFDC-PT-010食品中沙门氏菌检出能力验证。方法利用全自动免疫检测系统(VIDAS)对能力验证中的5个样品进行快速筛查,依据GB4789.4-2010沙门氏菌检验进行血清学试验,采用16S r DNA全序列分析、全自动微生物生化鉴定系统(VITEK2)和全自动微生物基因指纹鉴定系统(Ribo Printer)对分离出的疑似菌进行鉴定。结果编号为CODE 1的样品检出阿贡纳沙门氏菌和婴儿沙门氏菌,CODE 3检出蒙得维的亚沙门氏菌,CODE 5检出鼠伤寒沙门氏菌,CODE 2和CODE 4未检出。结论 5个样品测试均取得优秀的结果。

Objective To strengthen the detection capability of Salmonella in food and improve the competition ability of laboratory, and validate the capability of microorganism detection by ability test. The laboratory participated in proficiency testing on Salmonella detection in foods of NIFDC-PT-010 organized by National Institutes for Food and Drug Control. Methods Full automatic immunoassay system (VIDAS) was used for 5 samples rapid screening, Salmonella serology test was carried out according to GB4789.4-2010. The isolated bacteria of 5 samples were identified by 16S rDNA sequence analysis, automatic microbial, biochemical identification system (VITEK2) and automatic microbial genetic fingerprint identification system (RiboPrinter). ResultsSalmonella agone and Salmonella infants were detected in CODE 1, Salmonella Montevideo was detected in CODE 3, Salmonella typhimuriumwas detected in CODE 5, and Salmonella spp. was not detected in CODE 2 and CODE 4. Conclusion The test results of 5 samples were outstanding.