山药多糖得率-活性综合优化提取工艺

Optimization of extraction process for integration of polysaccharides yield and activity from Chinese yam

目的以山药为原材料,确定纤维素酶法提取山药多糖的最佳工艺。方法以山药多糖提取得率为评价指标,比较了提取温度、提取时间、pH值和加酶量对山药多糖得率的影响。在此基础上,通过正交试验进行4因素3水平的试验设计,以山药多糖提取得率、多糖总抗氧化能力及多糖得率-活性结合能力三项参数为指标,综合优化山药多糖的提取工艺。结果纤维素酶法提取山药多糖的最佳工艺为提取温度40℃、提取pH值4.5、加酶量2%、提取时间2 h。在此最优条件下,重复3次,多糖提取得率和多糖总抗氧化能力分别为3.370%和4.952 mg Vc/g。结论通过优化纤维素酶法提取工艺,山药多糖不仅得率较高,同时亦具有较强抗氧化活性。

Objective To optimize extraction process of polysaccharides from Rhizoma dioscorea(Chinese yam) by cellulose methods.Methods In order to evaluate the indicators,the effects of extraction temperature,extraction time,pH value and enzyme amount on yam polysaccharide yield were compared.With the yam polysaccharides yield,total antioxidant capacity and the yield-activity as the process evaluation indexes for the best extraction conditions were optimized by orthogonal experiment.Results The optimal extraction process included extraction temperature of 40 ℃,extraction pH 4.5,cellulose addition amount of 2%,and extraction time of 2 h.Under the optimal extraction conditions,the maximum yield of yam polysaccharides was 3.370%and the antioxidant capacity was 4.952 mg V_c/g.Conclusion By optimizing enzymatic cellulose extraction process,the polysaccharides obtained from Chinese yam not only have a higher yield,but also have a stronger antioxidant activity.