反相高效液相色谱和超高效液相色谱法测定饮料中咖啡因含量的比较研究

Comparison of reversed high performance liquid chromatography and ultra-high performance liquid chromatography on quantitative determination of caffeine in beverages

目的对原反相高效液相色谱(RP-HPLC)方法进行转化并优化,建立超高效液相色谱(UPLC)方法,为饮料中咖啡因的含量测定提供更快速、高效、灵敏和环保的方法。方法分别采用RP-HPLC法和UPLC法测定饮料中咖啡因的含量,并对比二者的精密度、稳定性、重复性和加标回收率。结果 2种方法所得8种市售饮料中咖啡因的含量测定结果变化趋势一致,但UPLC法含量测定结果比RP-HPLC法含量测定结果偏高,二者精密度、回收率均符合要求。结论 UPLC法可以成功替代RP-HPLC法,为饮料中咖啡因的快速定性定量分析与检测提供新的途径。

Objective To establish an ultra performance liquid chromatography (UPLC) method to replace the reversed high performance liquid chromatography (RP-HPLC) for a faster, more sensitive and more environmental friendly detection for caffeine content in beverages. Methods Both RP-HPLC and UPLC methods were used in the determination of caffeine content in 8 kinds of beverages, and the precision, stability, repeatability and recovery rates of the 2 methods were compared. Results Caffeine content determined in 8 beverages by the 2 methods had a similar trend, but the content of caffeine determined by UPLC was higher than that by RP-HPLC. Both methods showed good precision and recovery rates. Conclusion UPLC method as a new detection method can replace RP-HPLC in the quantitative analysis of caffeine in beverages.