食品中维生素检测技术研究进展

Research progress of the detection technology for vitamins in food

维生素是机体维持其正常生活所必需的一类营养素,摄入不足或过量均可导致机体功能障碍,分析和评价食品中的维生素含量将对指导人群科学摄取维生素具有重要参考价值。本文对近年来国内外食品中维生素的前处理技术和常用检测技术进行了综述。检测技术主要包括微生物法、光谱分析法、高效液相色谱法、高效液相色谱-串联质谱法和电化学方法等,介绍了各种检测技术的原理及特点,着重介绍了高效液相色谱法在维生素测定中的应用。高效液相色谱法是目前使用最普遍的测定维生素的方法,应用范围很广,灵敏度也高,具有样品前处理简单,样品用量少,分离速度快,可一次性分析多种维生素等优点。本文对目前食品中维生素检测存在的问题和今后的发展方向提出建议,为今后维生素的高通量快速分析提供文献参考。

ABSTRACT:Vitamins are kinds of organic compounds essential to health. Excessive and inadequate vitamins intake can both cause deviant metabolic function. The analysis and evaluation of the vitamins content in foods can provide necessary reference to the scientific intake of vitamins. This paper provided a comprehensive overview of various extract and analytical techniques for the detection technology of vitamins at home and abroad in recent years. Analytical techniques mainly included microbial method, spectrophotometry, high performance liquid chromatography method, high performance liquid chromatography-tandem mass spectrometry method and electrochemical method. The principle and characteristics of a variety of detection technology were discussed. The application of high performance liquid chromatography in the determination of vitamin was emphatically introduced. High performance liquid chromatography was the most commonly used method in the determination of vitamin. It had the advantages of simple sample pretreatment, less sample consumption, rapid separation, and can be used for analyzing many kinds of vitamins. The existing problems and the future development direction were suggested in light of the potentiality of technical novelty, which would provide references for further rapid simultaneous determination of multi-vitamins in food.