微生物法测定多种维生素片中的生物素含量

Microorganism determination of biotin content in multi-vitamins tablets

目的利用微生物法测定多种维生素片中生物素的含量。方法在GB 5413.19-2010分析方法的基础上,通过将乳酸杆菌培养基改成MRS肉汤培养基来制备菌悬液,样品经过65℃~70℃水浴超声提取,在630nm波长下用酶标仪测定培养液吸光度,对生物素含量进行定量检测。与国标法进行标准曲线、精密度、准确度对比试验,以及对新建立的方法进行加标回收试验。结果生物素浓度在0.01~0.1 ng/m L范围内与吸光度呈现良好的二次曲线关系,相关系数r2】0.999,精密度和准确度良好。在80%、100%、120%添加水平下,生物素的回收率分别为99.8%、96.1%、97.2%。结论方法操作简单、灵敏度高、处理量大,适合生产企业的多种维生素片的大批量质量控制和检测。

Objective To determine the content of biotin in multi-vitamins tablets by microbial assay. Methods On the basis of GB 5413.19 2010 analysis method, bacteria suspension were prepared by lactic acid bacteria culture medium to (MRS) broth culture medium, the sample was extracted by water bath ultrasonic after 65℃~70℃;then culture medium absorbance was measured by absorbance enzyme standard instrument under the 630 nm, and to quantitatively detect the content of biotin. The standard curve, precision and accuracy of contrast test were compared with the national standard method, and the new method had carried on the standard addition recovery test. Results Biotin concentration within the scope of 0.01 to 0.1 ng/mL and absorbance had a good conical relationship, the correlation coefficient r2>0.999, and had a good precision and accuracy. The recovery rates of biotin were 99.8%, 96.1%and 97.2%under adding level 80%, 100%, and 120%, respectively. Conclusion The method is simple, high sensitivity and large quantity, and it is suitable for the mass production enterprises of various vitamin pills quality control and testing.