红茶对高脂饮食小鼠肝脏的保护作用研究

Protective effects of black tea on mice liver under high fat diet

目的研究红茶对高脂饮食小鼠肝脏的保护作用。方法采用高脂饮食饲养小鼠,同时预防性给予不同产地、不同剂量的红茶(black tea,BT),分别测定小鼠的肝脏指数、肝脏丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、血清谷丙转氨酶(alanine transaminase,ALT)和谷草转氨酶(aspartate transaminase,AST)的含量,并通过肉眼和光学显微镜分别观察肝脏的病理变化。结果与高脂模型对照组相比,红茶剂量组血清ALT、AST、肝脏MDA、肝重及肝脏指数显示不同程度的降低,肝脏SOD及GSH-Px活力则有所上升,肝脏病变程度有一定程度的改善。结论结果表明红茶具有一定的保护和修护高脂饮食小鼠肝脏的作用,其机制可能与其能改善肝脏氧化应激状态、抑制脂质过氧化有关。

Objective To study the protective effect of black tea(BT) on mice liver under a high fat diet. Methods The mice were fed with BT of different types and quantities under the condition of high fat diet. Liver index and content of liver malondialdehyde(MDA), liver superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), serum glutamic-pyruvic transaminase(ALT) and glutamic-oxalacetic transaminase(AST) were measured, and the histopathological changes in liver tissues were observed with unaided eyes and under an optical microscope as well. Results The content of serum ALT, AST, liver MDA and liver index in the black tea feeding group were lower than the high hyperlipoidemia model group, while liver SOD activity and GSH-Px activity were higher, and the liver lesions were reduced. Conclusion It indicates that BT has positive effect on protecting and repairing mice liver with high fat diet. The mechanisms may be that black tea has effects on ameliorating oxidative stress and reducing lipid peroxidation product.