多种蜂胶中总黄酮检测方法的适用性研究

Applicability of methods for detection of total flavone in multiple propolis

目的通过3种分光光度法之间差异的比较和适用性考量,筛选出适合检测蜂胶中总黄酮含量的分光光度法。方法采用同批次样品,对蜂胶(国产蜂胶和巴西绿蜂胶)中总黄酮检测方法进行回收率和含量测定,并对结果进行分析。结果巴西绿蜂胶粉3种方法的检测结果为4.99~9.34 g/100 g;国产蜂胶粉3种方法的检测结果为7.82~17.97 g/100 g,其RSD为24.2%~31.2%;可见3种方法测定结果偏差较大。结论 GB/T20574-2006的方法最适合蜂胶中总黄酮的检测。

Objective To choose the best method for detection of the total flavone content in propolis by comparing the differences of 3 spectrophotometry methods. Methods The content of total flavone in the samples(domestic propolis and Brazilian green propolis) from the same batch was determined by the 3 different methods respectively and the results were analyzed. Results By different methods, the content of total flavone was 4.99~9.34 g/100 g in green propolis, and 7.82~17.97 g/100 g in domestic propolis, with the RSD of 24.2%~31.2%. There was a big deviation among the 3 methods. Conclusion The method from GB/T 20574-2006 is the best one to determine the total flavone content in propolis.