茶多酚急性毒性试验研究

Experimental study on acute toxicity of tea polyphenols

目的对茶多酚的急性毒性进行研究,为其在保健食品中的应用提供理论依据。方法将大鼠分为6个组,大鼠灌胃剂量分别为1.00、2.15、4.64、10.0、21.5 g/kg和空白对照组。按大鼠体重20 m L/kg给予各浓度的受试液灌胃,每天灌胃2次,连续灌胃14 d。结果高剂量茶多酚灌胃大鼠后相继出现死亡,低剂量组(1.00 g/kg、2.15 g/kg)大鼠于14 d后解剖观察,未发现明显异常;与空白对照组比较,大鼠脏器重和脏体比,无显著差异(P>0.05)。各剂量组大鼠体重与对照组比较,差异无统计学意义(P>0.05)。结论茶多酚属低毒级,在以后的生产应用中应该注意其用量。

Objective To study the acute toxicity of tea polyphenols in order to provide a theoretical basis for the application in health food. Methods The rats were divided into 6 groups, i.e., the five groups fed with the intragastric dose of 1.00, 2.15, 4.64, 10.0, 21.5 g/kg respectively and the blank control group. The rats were given test solutions of different concentrations by the weight capacity of 20 m L/kg, twice a day for consecutive 14 days. Results Successional death occurred in rats fed with high doses of polyphenols. No obvious abnormalities were observed in rats of low dose groups(1.00 g/kg, 2.15 g/kg) after dissection at 14 d. There were no significant differences in the organ weight and the ratio of organ to body weight compared with the control group(P>0.05). There was no statistical significance in the body weight in comparison of the rats in each dose group and the control group(P>0.05). Conclusion Tea polyphenols are at low toxicity level, and attentions should be paid to their dosage during the production and application of tea polyphenols in the future.