板栗苞中栲胶提取工艺的研究

Study on tannin extract from chestnut(Castanea mollissima Blume) shell

目的以板栗苞为原料,研究板栗苞提取栲胶的最佳工艺。方法在粉碎粒度、丙酮溶液浓度、料液比和超声功率单因素实验基础上,以栲胶提取率为响应值,设计4因素3水平的均匀实验,通过均匀设计偏最小二乘回归建模分析,得出最优指标时各个因素组合参数,并采用铬皮粉法对最优条件下提取的栲胶进行分析。结果板栗苞中栲胶提取的最佳工艺为:粉碎粒度100目,50%丙酮溶液,料液比1:18,超声功率400 W,在此条件下,栲胶提取率为31.28%,单宁含量为63.68%,p H值为3.81,总色值为22。结论以板栗苞为原料提取的栲胶达到了橡椀栲胶冷溶合格品的指标,说明板栗苞提取栲胶的工艺可行性。

Objective To obtain the optimum extraction parameters of tannin extract from chestnut shell. Methods Based on single-factor test, uniform experimental design with 4-factor and 3-level was adopted to study the effects ofparticle size, concentration of acetone solution, solid-liquid ratio and ultrasonic power on the extraction rate of tannin extract. Using partial least squares regression analysis, the optimal extraction parameters were obtained. Component of tannin extract was also analyzed by hide powder method.Results The optimum conditions of tannin extract from chestnut shell were as follows: particle size was 100 meshes, 50% acetone solution, solid-liquid ratio was 1:18 and ultrasonic power was 400 W. Under this condition, the extraction rate of tannin extract was 31.28%, the content of tannin was 63.68%, the pH value was 3.81 and the total color value was 22.Conclusion The tannin extract from chestnut shell has reached the standard of cold-soluble valonea extraction, which illustrated the feasibility of using chestnut shell as a kind of material for tannin extract.