高效液相色谱法同时检测肉制品中4种着色剂

Simultaneous determination of 4 colorants in meat products by high performance liquid chromatography

目的建立高效液相色谱法检测肉制品中诱惑红、胭脂红、柠檬黄、日落黄的实验方法。方法采用氨水-乙醇溶液提取,用正己烷脱脂,对氨水乙醇提取液采用定量浓缩仪浓缩。利用液相色谱作为检测手段,紫外检测器分波段采集。结果在0~50.0μg/m L范围内线性良好,4种色素的相关系数均大于0.999,平均回收率为诱惑红78.6%,胭脂红79.2%,柠檬黄78.3%,日落黄79.6%。检出限均为1.0 mg/kg。结论该方法具有样品预处理简单、快速、灵敏度高等特点,可用于肉制品中诱惑红、胭脂红、柠檬黄、日落黄的同时检测。

Objective To establish a method for the determination of 4 colorants(allure red AC, carmosine, tartrazine, sunset yellow FCF) in meat products by high performance liquid chromatography(HPLC). Methods The sample was extracted by the solution which was made up by ammonium hydroxide and ethanol and degreased by n-hexane, then concentrated by sample concentrator. The sample was detected by HPLC with UV detector. Results This method showed a good linearity in the range of 0~50.0 μg/m L(r2>0.999). The average recovery were 78.6%, 79.2%, 78.3%, and 79.6% for allure red AC, carmosine, tartrazine, and sunset yellow FCF, respectively. The detection limit was 1.0 mg/kg for all. Conclusion The method is simple, rapid and high sensitive, which can be used for the detection of allure red AC, carmosine, tartrazine, sunset yellow FCF in meat products.