高效液相色谱-柱后衍生法测定植物油脂中4种黄曲霉毒素

Determination of four kinds of aflatoxins in vegetable oil by high performance liquid chromatography with post column derivation

目的建立一种可同时测定植物油脂中黄曲霉毒素G2、G1、B2、B1残留量的分析方法。方法将待测样品经前期溶剂提取、免疫亲和柱净化处理后,通过高效液相色谱-柱后衍生-荧光检测器进行测定。结果黄曲霉素G2、B2在0.1~2 ng/m L范围内表现出良好的线性关系,而黄曲霉素G1、B1在1~20 ng/m L范围内r>0.9995,其线性关系良好。在添加浓度为0.1~20μg/kg的范围内,平均回收率在75.0%~125.7%之间。结论该方法快速简便准确,可作为植物油脂中黄曲霉毒素的残留量测定方法。

Objective To establish a simultaneous detection method for the determination of aflatoxin G2, G1, B2 and B1 residue in vegetable oil. Methods After solvent extraction and purification with immunoaffinity column, the oil samples were analyzed by high performance liquid chromatography-post column derivation-fluorescence detector(HPLC-PCD-FLD). Results The linear relationship of aflatoxin G2 and B2 ranged from 0.1~2 ng/m L, and of aflatoxin G1 and B1 ranged from 1~20 ng/m L was excellent, and r was larger than 0.9995. The average recovery rate was from 75.0% to 125.7% when the addition concentration ranging from 0.1 μg/kg to 20 μg/kg. Conclusion This method is simple and quick, and can be used for the determination of aflatoxins residue in vegetable oil.