液相色谱/四级杆飞行时间质谱分析烟酸芽胞杆菌发酵产物苯乳酸

Analysis on phenyllactic acid from Bacillus niacini based on liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry

目的对烟酸芽胞杆菌Bacillus niacini FJAT-14202来源的苯乳酸成分进行分析与检测。方法采用液相色谱/四级杆飞行时间质谱(liquid chromatography-hybrid quadrupole time-of-flight mass spectrometry)分析烟酸芽胞杆菌FJAT-14202发酵液中胞外代谢物的成分。利用Mass Hunter软件,对原始数据进行分子特征提取,通过Metlin代谢物质谱数据库检索比对获得代谢物的信息。结果在烟酸芽胞杆菌FJAT-14202发酵液中检测到811种代谢物,通过Metlin谱库搜索获得初步鉴定的有165种。其中,发现了具有生物活性的物质苯乳酸,其匹配得分达到96.52,占发酵液总代谢物相对含量的5.31%,保留时间分别为2.1057 min,精确质量数为166.0552。结论苯乳酸的发现为烟酸芽胞杆菌的开发与利用提供理论依据。

ABSTRACT:Objective To analyze and test on active substance fromBacillus niacini FJAT-14202. MethodsExtracellular metabolites ofBacillus niacini FJAT-14202 were analyzed by liquid chromatography- hybrid quadrupole time-of-flight mass spectrometry. Molecular feature extraction(MFE) and database retrieval were applied by Agilent Mass Hunter software, for the information overall metabolites.Results Totally 811 compounds were detected, and 165 of them were identified by library searching of Metlin database. The match score of phenyllactic acid was 96.52 and itsrelative content was 5.31%. The retention time of phenyllactic acid was about 2.1057 min, and its accurate mass was 166.0552.Conclusion The results provide the theory basis for development and utilization of phenyllactic acid fromBacillus niacini.