灵芝多糖脱色工艺研究

Study on decolorization process of Ganoderma lucidum polysaccharides

目的优化灵芝多糖脱色工艺,选取最优脱色方式。方法选取活性炭、壳聚糖、H_2O_2 3种脱色剂对灵芝多糖脱色处理,以脱色率和多糖保留率为指标,在单因素实验基础上,进行正交工艺优化。以2,2-二(4-叔辛基苯基)-1-苦肼基自由基(DPPH·)清除活性分析脱色后的灵芝多糖的抗氧化活性。结果活性炭对灵芝多糖的脱色率为67.72%,多糖保留率为72.12%;壳聚糖对灵芝多糖的脱色率为33.57%,多糖保留率63.00%;H_2O_2对灵芝多糖的脱色率为84.11%,多糖保留率为73.12%,综合考虑H_2O_2脱色效果最好,壳聚糖脱色法所得多糖的DPPH自由基清除能力最强。结论活性炭、壳聚糖、H_2O_2 3种脱色剂均可用于灵芝多糖脱色, H_2O_2效果较好,值得进一步的开发和利用。

Objective To optimize the decolorization process of Ganoderma lucidum polysaccharides and select the best decolorization method. Methods Activated carbon, chitosan and H2O2 were selected for decolorize Ganoderma lucidum polysaccharides. Based on the single factor experiment, decolorization process was optimized by the orthogonal experiment, with the decolorization rate and the polysaccharide retention rate as indicators. The antioxidant activity of the decolorized Ganoderma lucidum polysaccharides was analyzed by the 2,2-bis(4-tert-octylphenyl)-1-picrylyl radical(DPPH·) scavenging activity. Results The decolorization rate of activated carbon on Ganoderma lucidum polysaccharides was 67.72%, the polysaccharide retention rate was 72.12%;the decolorization rate of chitosan on Ganoderma lucidum polysaccharides was 33.57%, the polysaccharide retention rate was 63.00%;the decolorization rate of H2O2 on Ganoderma lucidum was 84.11%, and the polysaccharide retention rate was 73.12%. H2O2 had the best decolorization effect. The polysaccharides obtained by chitosan decolorization had the strongest scavenging capacity for DPPH free radical. Conclusion Activated carbon, chitosan and H2O2 can be used for decolorization of Ganoderma lucidum polysaccharides, and the effect of H2O2 is much better, which is worthy of further development and utilization.