基于液相色谱-质谱的蛋白质组学技术在食品鉴伪中的应用

Application of proteomics technology based on liquid chromatography-mass spectrometry in food identification

随着全球化的发展,食品供应链范围更加宽泛,而食品掺假等欺诈问题日益严重,影响着全球的经济市场、食品质量与安全监管和消费者的身体健康,食品掺假现象已得到社会各界的广泛关注。食品检测技术错综复杂,随着人类基因组序列测定的完成,人们把检测的研究方向转向了蛋白质组学,这种检测手段的先进性、适用性在食品质量和安全等方面取得广泛应用。质谱作为蛋白质组学方法的常用技术,可利用质谱的高通量分析优势对蛋白质和肽段进行鉴定、检测和定量研究,即可开发简单的、快速的方法来分析复杂的或深加工的食物,又可定量检测具有高分离度的微量分析物。本文主要介绍了蛋白质组学方法在食品鉴伪中的应用,阐述了高分辨质谱技术在乳制品、肉类、海产品以及葡萄酒的食品欺诈检测鉴别中的运用,为该类食品安全问题的处理提供有效的参考。

With the development of globalization, the scope of food supply chain is more extensive, and food adulteration and other fraud problems are increasingly serious, which affects the global economic market, food quality and safety supervision and consumer health, food adulteration phenomenon has been widely concerned by the community. Food detection technology is complex. With the completion of human genome sequencing, people have turned the research direction of detection to proteomics. The advanced and applicability of this detection method has been widely used in food quality and safety. As a common technique of proteomics, mass spectrometry can be used to identify, detect and quantitatively study proteins and peptides by using its advantage of high throughput analysis. It can be used to develop simple and rapid methods for the analysis of complex or highly processed foods, and quantitative detection of trace analytes with high degree of separation. This paper mainly introduced the application of proteomics method in food adulteration, and expounded the application of high resolution mass spectrometry technology in the detection and identification of food fraud in dairy products, meat, seafood and wine, so as to provide an effective reference for the treatment of such food safety problems.