高效液相色谱-四极杆飞行时间质谱联用仪测定小鼠钩吻素子中毒模型的研究

Determination of koumine poisoning model in mice by high performance liquid chromatography-quadrupole time of flight mass spectrometer

目的通过动物急性钩吻素子染毒实验,建立一种测定小鼠钩吻素子的高效液相色谱-四极杆飞行时间质谱联用方法。方法小鼠经钩吻浸泡药酒染毒后取材,其血液、胃液、尿液通过1%甲酸乙腈沉淀蛋白后,进行上机分析。采用5 mmol/L乙酸铵-0.1%甲酸(A)和乙腈(B)作为流动相进行梯度洗脱,质谱正离子化信号采用母离子模式和子离子模式对钩吻素子进行监测。结果该实验结果显示1 h取材的动物样本,经过分析,胃液与尿液的差异性更大,且胃内容物钩吻素子含量较高。结论应急采样中,优先提供胃液物进行检验,其次到尿液。

Objective Through the animal acute leucin poisoning experiment, to establish a method for the detection of koumine in animals by high performance liquid chromatography-quadrupole time of flight mass spectrometer(HPLC-QTOF-MS). Methods After mice were poisoned with medicinal liquor, the protein in blood,gastric juice and urine of the mice were precipitated through 1% acetonitrile, and then analyzed by HPLC-QTOF-MS.Ammonium acetate-0.1% formic acid(A) and acetonitrile(B) were used as mobile phases for gradient elution.Koumine were monitored by mass spectrometry electrospray ionization(positive) using the mother ion model and daughter ion model. Results The results showed that the difference between gastric juice and urine was greater, and the koumine content of gastric contents was higher. Conclusion In emergency sampling, the first priority is to provide gastric juice for testing, followed by urine.