2015～2018年北京市食源性金黄色葡萄球菌耐药性分析

Surveillance on antibiotic resistance of food-borne Staphylococcus aureus in Beijing from 2015 to 2018

目的掌握北京市食源性金黄色葡萄球菌对常用抗生素的耐药谱和耐药趋势。方法收集2015?2018年北京市食源性致病菌监测网分离到的金黄色葡萄球菌103株,采用CLSI推荐的微量肉汤稀释法检测金黄色葡萄球菌对11种常用抗生素的敏感性。结果 103株菌中有102株表现为对至少1种抗生素耐药,耐药率排在前三位的是青霉素、红霉素和克林霉素,其中对青霉素的耐药率最高,为99.0%。检出耐甲氧西林金黄色葡萄球菌8株,占7.8%,未见万古霉素耐药株。45.6%(47/103)的菌株同时耐受3类和3类以上抗生素,表现为多重耐药,同时耐受抗生素种类最高为7类。共存在26种耐药谱,优势耐药谱为青霉素、青霉素-红霉素-克林霉素-复方新诺明-庆大霉素和青霉素-红霉素。结论 2015～2018年北京市食源性金黄色葡萄球菌整体耐药水平较高,且多重耐药情况严重,并出现MRSA株,应继续加强北京市食源性金黄色葡萄球菌的耐药性监测。

Objective To investigate the trend of antibiotic resistance and antibiotic resistance spectrum of foodborne Staphylococcus aureus in Beijing.Methods Totally 103 Staphylococcus aureus stains isolated form foodborne pathogenic bacteria monitoring network in Beijing from 2015?2018 were tested against 11 commonly used antibiotics using broth micro-dilution method recommended by CLSI.Results The 102 strains of 103 strains of Staphylococcus aureus showed resistance to at least 1 antibiotic.Resistance rates to penicillin,erythrocin and clindamycin ranked in the top three,among which penicillin had the highest resistance rate(99.0%),8 methicillin-resistant Staphylococcus aureus strains were detected,accounting for 7.8%,vancomycin-resistant strains were not found.A total of 45.6%(47/103)of them were identified as multiple antibiotic resistance strains and were resistant to as much as 7 classes of antibiotics.There were 26 antibiotic resistance spectrums with penicillin,penicillin-erythrocin-clindamycin-trimethoprim-sulfamethoxazole-gentamicin and penicillin-erythrocin as the top three spectrums.Conclusion From 2015 to 2018,the overall level of drug resistance of foodborne staphylococcus aureus in Beijing is relatively high,and the situation of multiple drug resistance is serious,and MRSA strain appears,so the monitoring of drug resistance of foodborne staphylococcus aureus in Beijing should be further strengthened.