响应面法优化铜藻中岩藻黄素的提取工艺

Optimization of extraction process for fucoxanthin from Sargassum horneri by response surface methodology

目的采用响应面法优化铜藻中岩藻黄素的乙醇溶液提取工艺。方法用乙醇溶液作提取溶剂,采用超声法提取铜藻中的岩藻黄素,研究乙醇浓度、料液比、提取温度、提取时间对岩藻黄素提取的影响,在单因素试验的基础上,用Box-Benhnken中心组合试验设计迚行4因素3水平的响应面分析,以岩藻黄素的提取量为响应值,优化提取工艺参数。结果响应面试验得到超声提取最佳条件:料液比1:100、起始温度60℃、超声时间40 min、乙醇浓度70%。在优化的条件下,超声提取岩藻黄素提取量为1092μg/g,和预测值1183μg/g基本一致。结论通过响应面优化,得到了乙醇溶液超声提取铜藻中岩藻黄素的最佳条件,提高了岩藻黄素的提取量。

Objective To optimize the extracting conditions of fucoxanthin from Sargassum horneri by response surface methodology(RSM).Methods Fucoxanthin was extracted from Sargassum horneri with ethanol as solvent by ultrasonication.The effects of ethanol concentration,solid-liquid ratio,extraction temperature and time on the extraction of fucoxanthin from Sargassum horneri were studied.Based on the results of single factor experiments,Box-Benhnken design center combination method of 4 factors and 3 levels experimental design was applied to further optimize the extraction conditions using the extraction yield as response value.Results The response surface interaction analysis showed that the optimum extraction conditions for ultrasonic extraction were solid-liquid ratio at1:100,initial temperature at 60℃,extraction time at 40 min and ethanol concentration at 70%.Under the optimized conditions,the extraction yield of fucoxanthin from Sargassum horneri by ultrasonic extraction was up to 1092μg/g,which was highly consistent with the predictive value of 1183μg/g.Conclusion By response surface optimization,the optimum conditions for ultrasonic extraction of fucoxanthin from Sargassum horneri by ethanol solution were obtained,which increased the extraction amount of fucoxanthin.