实时荧光PCR法测定婴幼儿谷类辅助食品中麸质过敏原成分

Determination of gluten allergens in cereals of supplementary foods for infants and young children by real-time fluorescent PCR method

目的建立实时荧光PCR法检测婴幼儿谷类辅助食品中麸质过敏原成分的含量。方法样品加入淀粉酶液化后,采用试剂盒方法提取样品DNA,考察大麦Hordein基因、小麦Gliadin基因、黑麦Secl基因和燕麦Avenin基因检测方法的特异性、灵敏度和检出限,幵应用于检测实际样品。结果确定了实时荧光PCR反应体系条件,各个基因的检测方法具有特异性强且灵敏度高,检出限为0.1%。结论该方法操作简便,准确率高,适用于婴幼儿谷类辅助食品中麸质成分的检测。

Objective To establish a method for the determination of gluten allergens in cereal supplement foods for infants and young children by real-time fluorescent PCR method.Methods After the sample was liquefied by amylase,the sample DNA was extracted by kit method.The specificity,sensitivity and detection limit of the detection method of Hordein gene,wheat Gliadin gene,rye Secl gene and oat Avenin gene were investigated and applied to the detection of actual sample.Results The conditions of the real-time fluorescent PCR reaction system were determined.The detection methods of each gene were specific and sensitive,and the detection limit was 0.1%.Conclusion This method is simple,accurate and suitable for the detection of gluten allergens in cereal supplement foods for infants and young children.