紫外串联荧光检测法测定奶昔中维生素B_6和烟酰胺

Determination of vitamin B_6 and nicotinamide in milk shake by ultraviolet tandem fluorescence method

目的建立紫外串联荧光检测法同时快速检测奶昔中维生素B_6和烟酰胺的分析方法。方法参照GB 5009.154-2016《食品中维生素B_6的测定》和GB 5009.89-2016《食品中烟酸和烟酰胺的测定》,采用带有紫外检测器和荧光检测器的高效液相色谱仪快速检测奶昔中维生素B_6和烟酰胺。结果该方法的准确度偏差<15%,线性相关系数>0.99,重复性和稳定性均符合标准要求,平均回收率在95%～105%之间,均符合标准GB/T 27404-2008《实验室质量控制规范食品理化检测》中的要求,且目标峰分离效果更好,效率提升49.5%。结论该方法的检测效率和分离效果较好,符合标准要求。

Objective To establish a method for rapid and simultaneous determination of vitamin B6 and nicotinamide in milk shake by ultraviolet tandem fluorescence method.Methods According to GB 5009.154-2016 Determination of vitamin B6 in foods and GB 5009.89-2016 Determination of niacin and nicotinamide in foods,vitamin B6 and nicotinamide in milk shake was determined by high performance liquid chromatography with UV detector and fluorescence detector.Results The accuracy deviation of this method was less than 15%,and the linear correlation coefficient was more than 0.99.The repeatability and stability were in line with the standard requirements,and the average recovery rate was between 95%-105%,which met the requirements of the standard GB/T 27404-2008 Laboratory quality control standard for food physical and chemical testing.The separation of target peaks was better,and the efficiency was increased by 49.5%.Conclusion The established method has better efficiency and separation effect,which can meet the standard requirements.