菌落总数质控样的制备及其在能力验证中的应用

Preparation of the quality control samples for aerobic plate count and the application in the proficiency test

目的制备均匀性和稳定性符合能力验证要求的质控样品,并将质控样品用于菌落总数的能力验证。方法本次考核样品由高浓度(3×10~4 CFU)、低浓度(1×10~4 CFU)2个样品组成,随机抽取质控样品进行稳定性及均匀性的检验,对其进行评价。本设计通过对实验室进行随机分组来发放样品,防止能力验证活动中各实验室进行串通。结果 65家实验室参加本次考核活动,整体满意率为80%,本次能力验证可以真实地反映参试单位的检测水平。结论本次菌落总数能力验证样品可用于质控样品使用。

Objective To prepare quality control samples with uniformity and stability those meet the requirements for proficiency testing,and verify the ability of the quality control samples for the total number of colonies.Methods The test samples were composed of 2 samples with high concentration(3×10~4 CFU)and low concentration(1×10~4 CFU).Randomly selected samples were tested the stability and uniformity.This design distributed samples by randomly grouping laboratories to prevent collusion in laboratories in proficiency testing activities.Results Totally 65 laboratories participated in this proficiency test,80%of the laboratories with excellent results.This proficiency test could truly reflect the detection level of the participating units.Conclusion This verification samples of total number of colony can be used for quality control samples.