氨基酸分析仪检测婴幼儿奶粉中牛磺酸含量

Determination of taurine content in infant milk powder by amino acid analyzer

目的建立一种氨基酸分析仪测定婴幼儿奶粉中牛磺酸含量的分析方法。方法样品经0.01mol/L盐酸溶液溶解并用106 g/L亚铁氰化钾溶液和219 g/L乙酸锌沉淀蛋白质后10000 r/min离心10 min取出,精密取上清液置20mL量瓶中,用上样溶液(pH2.2)定容后,采用氨基酸分析仪钠系统进行程序洗脱,LCA K06/Na色谱柱进行分离,茚三酮溶液衍生显色,在570nm波长下测得牛磺酸色谱峰,用外标法峰面积定量,保留时间定性。结果牛磺酸在5.544~110.880μg/mL浓度范围内有良好的线性关系(r=1.0000),回收率为101.47%,相对标准偏差为1.6%(n=9)。结论该方法简单、便捷、准确性高,适合于婴幼儿奶粉中牛磺酸的定量分析。

Objective To establish an method using amino acid analyzer for the determination of taurine in infant milk powder.Methods The sample was dissolved in 0.01 mol/L hydrochloric acid solution and precipitated with 106 g/L potassium ferrocyanide solution and 219 g/L zinc acetate.The protein was centrifuged at 10000 r/min for 10 min,and the supernatant was accurately taken in a 20 mL volumetric flask.After constant volume with a sample solution(pH 2.2),the program was eluted with an amino acid analyzer sodium system,separated by LCA K06/Na column,and colorimetrically derived from ninhydrin solution,and the taurine chromatographic peaks was measured at a wavelength of 570 nm,quantification of peak area by external standard method,retention time qualitative.Results Taurine had a good linear relationship in the concentration range of 5.544-110.880 g/mL(r=1.0000)with a recovery rate of 101.47%and a relative standard deviation of 1.6%(n=9).Conclusion This method is simple,convenient,accurate and suitable for the quantitative analysis of taurine in infant milk powder.