东紫苏致突变作用的研究

Study on mutagenicity of Elsholtzia bodinieri Vaniot

目的研究东紫苏是否具有致突变作用。方法采用平板掺入法Ames试验,设每皿5000、1000、200、40、8μg剂量组,同时设阴性、阳性对照组,观察每皿回变菌落数。微核及精子畸形试验设5000、2500、1250 mg/kg·BW 3个剂量组、阴性对照组及阳性对照组。微核试验采用30 h、2次给受试物法,检测骨髓嗜多染红细胞微核率。精子畸形试验于首次染毒后第35 d处死动物,观察精子畸形率。结果与阳性对照组比较,东紫苏各剂量组在Ames试验中未呈现致突变性,骨髓细胞微核试验及小鼠精子畸变试验结果均为阴性,差异均无统计学意义(P>0.05)。结论在本实验条件和剂量范围内,未发现东紫苏有致突变性作用。

Objective To study the potential mutagenicity of Elsholtzia bodinieri Vaniot.Methods The plate incorporation Ames test was used to observe the number of colonies in each plate at the dose of 5000,1000,200,40,8μg/plate;and both negative and positive control were included in the assay.Bone marrow cell micronucleus test and sperm abnormality test were performed in a total of 3 dose groups(5000,2500,1250 mg/kg·BW),negative control group and positive control group.Micronucleus test was used to detect the micronucleus frequency of bone marrow polychromatic erythrocytes at 30 h after giving the target for twice.Sperm deformity test was performed on the thirty-fifth day after the first sample and sperm abnormality rate was calculated.Results Compared to the positive control group,the treatment group showed an absence of mutagenicity in the Ames test.The results of micronucleus test of bone marrow cells and the sperm aberration test in mice were negative,and no statistical significance was found(P>0.05).Conclusion Under the experimental conditions and the designed dose range,Elsholtzia bodinieri Vaniot showed no mutagenesis.