超高效液相色谱–质谱联用法定量测定奶茶中的3种毒品成分

Quantitative determination of 3 related narcotic substances in milk tea by ultra performance liquid chromatography-tandem mass spectrometry

目的建立超高效液相色谱质谱联用法测定奶茶中苯丙胺、甲基苯丙胺和氯胺酮的分析方法。方法样品经甲醇超声提取处理,利用超高效液相色谱质谱联用法, 4.0 min内完成样品的上机分析。结果方法在0.1~50.0 ng/mL范围内,线性良好,相关系数r大于0.999。仪器检出限在0.008~0.027 ng/mL之间,定量限在0.028~0.089 ng/mL之间。三水平加标回收率在80.7%~112.0%之间。高浓度样品分析后, 3种毒品无明显仪器残留。使用本方法分析市售奶茶样品,未检出3种毒品。结论本方法灵敏、快速、线性范围宽、选择性好、前处理简单,适用于奶茶中3种毒品的准确定量检测。

Objective To establish a method for determining amphetamine,methamphetamine and ketamine in milk tea by ultra performance liquid chromatography-tandem mass spectrometry.Methods The sample was ultrasonically extracted by methanol,utilizing ultra performance liquid chromatography-mass spectrometry,the sample analysis of the sample was completed within 4.0 min.Results In the range of 0.1-50.0 ng/mL,the linearity was good,and the correlation coefficient r was greater than 0.999.The limit of detection of the instrument was in 0.008-0.027 ng/mL,and the limit of quantification was in 0.028-0.089 ng/mL.The recoveries rate of the 3 levels was in 80.7%-112.0%.After analyzing the high-concentration samples,there were no obvious instrument residues in the 3 drugs.The method was used to analyze commercially available milk tea samples,and the 3 drugs were not detected.Conclusion This method is sensitive,rapid,wide in linear range,good in selectivity,simple in pretreatment,and suitable for accurate quantitative detection of 3 drugs in milk tea.