顶空气相色谱法快速测定酱油中丙酸盐的含量

Rapid determination of propionate in soy sauce by headspace gas chromatography

目的建立顶空技术串联气相色谱法快速检测酱油中丙酸盐的含量。方法准确称取一定量酱油至顶空品瓶内,加入磷酸使丙酸盐酸化为丙酸,采用ZB-WAXplus毛细管柱(30 m×0.25 mm, 0.25μm)分离,火焰离子检测器检测器检测,外标法定量。结果丙酸盐在10~200μg/mL浓度范围内线性关系良好,相关系数为0.9992,检出限为0.02 g/kg,三水平加标回收率为97%~107.5%,相对标准偏差为1.95%~2.63%。结论该方法前处理简单,检测结果可靠准确,适用于酱油中丙酸盐的含量的测定。

Objective To establish a method for rapid determination of propionate in soy sauce by headspace tandem gas chromatography.Methods A certain amount of soy sauce were accurately weighed into the headspace bottle and propionic acid was converted into propionic acid by adding phosphoric acid.The samples were separated with a ZB-WAXplus capillary column(30 m×0.25 mm,0.25μm),detected by flame ion detector detector and quantitative analyzed by external standard method.Results Propionate had a good linear relationship in the range of 10-200μg/m L,and the correlation coefficients was 0.9992.The limit of detection was 0.02 g/kg.The recoveries at 3 spiked levels were 97%-107.5%,with the relative standard deviations of 1.95%-2.63%.Conclusion The method has simple pretreatment and reliable and accurate detection results,and is suitable for determination of propionate content in soy sauce.