微波水解-茚三酮柱后衍生离子交换色谱法测定鹌鹑蛋中的17种氨基酸

Determination of 17 kinds of amino acids in quail eggs by microwave hydrolysis-ninhydrin column post-derivative ion exchange chromatography

目的建立微波水解茚三酮柱后衍生离子交换色谱法同时测定鹌鹑蛋中17种氨基酸含量的分析方法。方法采用磺酸型阳离子树脂离子交换柱分离,茚三酮溶液作为柱后衍生剂,紫外可见分光光度检测器进行检测,检测波长为570nm和440nm,并对微波水解时间及温度进行条件优化。结果 17种氨基酸在一定范围内呈良好线性关系,相关系数均大于0.99;检出限为0.376~0.906mg/L。结果表明,150℃下微波水解20 min的结果与传统加热水解(110℃, 22 h)的效果基本相同。结论该方法分析时间较短,灵敏度较高,可用于鹌鹑蛋中氨基酸含量的检测。

Objective To establish a method for simultaneous determination of 17 amino acids in quail eggs by microwave-hydrolyzed ninhydrin column post-derivative ion exchange chromatography.Methods The sulfonium-type cationic resin ion exchange column was used for separation,and the ninhydrin solution was used as a post-column derivatizer.The UV-visible spectrophotometer was used for detection.The detection wavelengths were 570 nm and 440 nm,and the microwave hydrolysis time and temperature were optimized.Results The 17 amino acids showed a good linear relationship in the range of 0.005-1.000μmol/mL,and the correlation coefficient was greater than 0.99.The limits of detection were 0.376-0.906 mg/L.The results of microwave hydrolysis at 150℃for 20 min were basically the same as those of traditional heating hydrolysis(110℃,22 h).Conclusion This method has short analysis time and high sensitivity,and can be used for detecting the amino acid content in quail eggs.