摘要
采用聚乙二醇/硫酸铵体系对生物胺氧化酶进行萃取。通过聚乙二醇的分子质量和质量分数、(NH4)2SO4的质量分数、体系p H值以及温度单因素实验,初步确定最优值,再用Box-Behnken响应面进一步优化实验并进行结果分析,得到最佳实验条件为:质量分数为25%的PEG2000、质量分数为10%的(NH4)2SO4、p H值为7.5、温度为30℃,实验的萃取率为96.5%,纯化倍数为2.38。
PEG /( NH4)2SO4aqueous-phase systems was used to extract biogenic amine oxidase that produced by Lactobacillus rhamnosus. Based on the single factor experiments including PEG molecular mass and concentration,ammonium sulfate concentration,p H value and temperature,preliminarily determine the optimum values. Then the design was further optimized with Box-Behnken experiment and the results were analyzed. The results indicated that the best experiment conditions were as follows: PEG molecular mass was 2000,PEG2000 concentration was 25%,ammonium sulfate concentration was 10%,p H value was 7. 5,and the temperature was 30 ℃. Under above conditions,the extraction rate was 96. 5% and the purification factor was 2. 38.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第3期156-162,共7页
Food and Fermentation Industries
基金
国家自然基金
熏马肠中生物胺产生和累积机理的研究(No.31160329)
产胺氧化酶发酵剂对新疆熏马肠中生物胺影响及其作用机制(No.31360392)
新疆生产建设兵团博士基金
强化接种调控内源酶在熏马肠中应用研究(No.2013BB012)
关键词
聚乙二醇/硫酸铵
鼠李糖乳杆菌
生物胺氧化酶
双水相
萃取
PEG /(NH4)2SO4
extraction
Lactobacillus rhamnosus
biogenic amine oxidase
two aqueous-phase systems
