金银花绿原酸和木犀草苷的提取

Optimization of extraction technology of chlorogenic acid and cynaroside from honeysuckle

为确定金银花中绿原酸和木犀草苷同时提取的最佳工艺条件,以两者得率为指标,在单因素试验的基础上,采用响应面法对主要工艺参数进行优化并得到回归模型。结果表明:回归模型能较好地反映指标与工艺参数之间的关系;最佳工艺条件为提取时间1.25 h,温度71℃,乙醇浓度体积分数60%,料液比1∶25(g∶m L),此条件下绿原酸得率为3.741%,木犀草苷得率为0.067%,与模型预测值相近。

In order to determine the optimum extracting condition of chlorogenic acid and cynaroside from honeysuckle,response surface methodology was applied to obtain primary parameters and a regression mode was established based on the single factor experiment using their yields as indicators. Results showed that the regression mode properly reflected relationships among indicators and parameters; the optimum extraction condition was 1. 25 h,temperature71℃,alcohol concentration 60%( V / V) and liquid-to-material 1 ∶ 25( g∶ m L). Under the optimum extraction condition,the yield of chlorogenic acid and cynaroside were 3. 741% and 0. 067% respectively and was close to the predicted values.