补充营养物对硅橡胶膜生物反应器长期封闭循环发酵性能的影响

Effect of nutrient supplement on long time closed-circulating fermentation in PDMS membrane bioreactor

通过向发酵液中补充营养物质,研究了补充营养物对膜生物反应器长期封闭循环发酵行为的影响;实验得到的细胞浓度为7.10 g/L,细胞存活率为0.90,发酵液内的平均乙醇浓度为68.6 g/L,乙醇产率为1.85 g/(L·h),乙醇得率为0.411 g/g,乙醇比产率为0.286 g/(g·h),与不补充营养物质的实验相比,发酵性能有所提高;实验发现补充营养物质,没有提高细胞浓度和细胞存活率,而是增强了细胞的生命活性,提高了细胞产乙醇的能力。

The effects of nutrient supplement on long time closed-circulating fermentation in PDMS membrane bioreactor were studied by supplementing nutrient to broth during fermentation. In the test of nutrient supplement,the concentrations of cell and ethanol were 68. 6 g / L and 7. 10 g / L while the cell viability and the ethanol productivity were 0. 91 and 1. 85 g /( L·h). The results indicated that the cell concentration and viability did not increased by nutrient supplement. The specific ethanol production and ethanol yield were 0. 286 g /( g·h) and 0. 411 g / g,which were higher than those without nutrient supplement,suggesting that the producing ethanol ability of the cells were improved. The results showed the performance of fermentation in the membrane bioreactor could be improved by supplementing nutrient to broth.