培养基质对蛹虫草中虫草酸及核苷类物质的影响

Effects of culture medium on cordycepic acid and nucleosides in Cordyceps militaris

采用燕麦、小米、大麦、大米4种培养基质固体培养获得蛹虫草子座,比较子座中虫草酸、虫草素、腺苷、尿苷、鸟苷及胸苷含量的差异。采用比色法测定虫草酸含量,采用高效液相法测定核苷类物质含量。结果表明:培养基质对蛹虫草代谢产物合成的影响各有不同,小米培养的子座虫草酸(9 000μg/g)及虫草素(9 531.0μg/g)含量最高,大米培养的子座腺苷(818.9μg/g)和尿苷(2 028.4μg/g)含量最高,大麦培养的子座鸟苷(1 105.3μg/g)含量最高。

The content of cordycepic acid, cordycepin, adenosine, uridine, guanosine and thymidine in Cordyceps militaris stromata cultured by Oat,millet,barley,rice,4 kinds of solid culture medium,were compared to obtain differences. The colorimetry method and the high performance liquid chromatography method were separately used to determine the content of cordycepic acid and nucleosides. The results showed that the effects of culture medium on the synthesis of metabolites in Cordyceps militaris were different. The content of cordycepic acid( 9 000 μg / g)and cordycepin( 9 531. 0 μg / g) in stroma cultured by millet were the highest. Adenosine( 818. 9 μg / g) and uridine( 2 028. 4 μg /g) in stroma cultured by rice were the highest. Stroma cultured by barley had the highest content of guanosine( 1 105. 3 μg / g). The research results provide references for the choice of culture medium of Cordyceps militaris.