摘要
采用肾上腺素结合冰浴的方法制备血管内皮细胞损伤的动物模型,并用免疫荧光法观察循环内皮细胞数,用酶联法测定血浆组织纤溶酶原激活物(t-PA)、抗凝酶Ⅲ(AT-Ⅲ)、6-酮-前列腺素E1α(6-keto-PGE1α)和血栓素B2(TXB2)的含量。结果表明:与模型组相比,榛蘑多糖可减少循环内皮细胞数,不同程度地提高t-PA和AT-Ⅲ,同时减少TXB2的含量,对6-keto-PGE1α的影响不明显。说明榛蘑多糖具有一定的血管内皮细胞保护作用。
To study the effect of polysaccharides from Armillaria mellea on endothelial cell injury in rats. Methods: Animal model was established by giving rats an injection of adrenaline and making it swim in ice-cold water. The number of circulating endothelial cells was measured with the immunofluorescence method,The levels of t-PA,AT-Ⅲ,6-keto-PGE1α and TXB2 in plasma was measured by ELISA method. Results: Armillaria mellea polysaccharides decreased the number of circulating endothelial cells,increased the levels of t-PA and AT-Ⅲ,reduced TXB2 content as compared with the model group. However,there was no significant difference in the 6-keto-PGE1α level. Conclusion: Polysaccharides from Armillaria mellea present the protective effect on vascular endothelial cells.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第5期157-159,共3页
Food and Fermentation Industries
基金
国家自然科学基金资助项目(No.30760150
No.81360088)
吉林省科技发展计划项目(20140101223JC)
