响应面法优化酶辅助微波提取四方藤总香豆素工艺

Optimization of Enzyme-Assisted Microwave Extraction of Total Coumarins from Cissus pteroclada Hayata by Response Surface Methodology

为优化酶辅助微波提取四方藤总香豆素工艺,在单因素试验基础上,应用中心组合设计,采用二次多项式逐步回归分析对提取工艺条件进行优化,确定酶辅助微波提取四方藤总香豆素的工艺最佳条件:酶用量0.5%, pH5.1,酶解时间1.5 h,微波萃取时间9 min;在此工艺条件下,四方藤总香豆素的提取率可达13.68%。

Enzyme-assisted microwave extraction of total coumarins from Cissus pteroclada Hayata was optimized by using single factor experiment and central composite design combining quadratic polynomial stepwise regression analysis. It was concluded that the optimal condition of extraction was enzyme dosage 0.5%, pH 5.1, enzymolysis time 1.5 h and microwave extraction time 9 min. Under these conditions, the yield of total coumarins in Cissus pteroclada Hayata was up to 13.68%.