超声-低温双水相提取雨生红球藻中的虾青素

Ultrasonic-Hypothermic Aqueous Phase Extraction of Astaxanthin from Haematococcus pluvialis

通过超声-低温破壁手段集合双水相技术对雨生红球藻进行破壁。以温度和提取率为参照对象确定超声波占空比为33%。构建叔丁醇-盐双水相体系,利用单因素试验确定影响因素,并对影响因素条件进行正交试验的设计和优化。影响雨生红球藻中虾青素提取的条件强弱为:提取次数>提取时间>醇水比值>料液比值。醇水比值1.3,料液比值0.015 (g/mL),提取时间70 min,提取次数3次为最佳提取条件,在最佳提取条件下提取虾青素的提取率可达4.08%。结合SEM图表征,可明显观察到雨生红球藻被破壁前后的形貌状态,可大致看到雨生红球藻的壁厚约410 nm,实现可视化分析。

The Haptococcus pluvialis was disrupted by the ultrasonic-low temperature wall-breaking technique and the dual aqueous phase technique.A tert-butanol-salt two-phase aqueous system was constructed.Through the single factor experiment,the experimental factors were ensured.The orthogonal experiments were designed and optimized for the influencing factors.The parameters affecting the extraction of astaxanthin from Haematococcus pluvialis were that the extraction times>extraction time>alcohol-water ratio>solid-liquid ratio.The best extraction condition was that the ratio of alcohol to water 1.3,the ratio of material to liquid 0.015(g/mL),extraction time 70 min,and extracting 3 times.The extraction rate of astaxanthin extracted under optimal extraction condition was up to 4.08%.The Haematococcus pluvialis could be more effectively presented by the optimized condition.After the tert-butanol was extracted and dissolved,the freeze-dried product was freeze-dried with a vacuum freeze dryer to obtain dried astaxanthin.Combined with the SEM image characterization,the morphology of the Haematococcus pluvialis before and after breaking was clearly observed.It could be seen that the Haematococcus pluvialis had a wall thickness of about 410 nm for visual analysis.