番茄皮中番茄红素含量测定方法

Determination Method of Lycopene Content in Tomato Skin

番茄皮是番茄红素工业化生产的重要原料来源。试验研究了番茄皮中番茄红素含量的质量评价方式,对比验证了不同干燥方式和萃取方式对检测结果的影响情况。结果表明:低温干燥、真空干燥和冷冻干燥均不会造成番茄皮中番茄红素损失;番茄皮粉可以使用正己烷、丙酮、乙酸乙酯或二氯甲烷直接进行萃取;番茄皮浆可以先通过甲醇或无水乙醇洗涤脱水,再用正己烷进行萃取,测定洗涤液和萃取液,再合并测定结果。以上几种处理方法均可以作为评价番茄皮中的番茄红素含量的方法。

Tomato skin is the main industrial material source to product lycopene.The way to evaluate the content of lycopene in the tomato skin was studied.Different methods of drying and extraction ware used to contrast the influence of the lycopene result.The results show that cold drying,vacuum drying and freeze drying will not damage the lycopene in the tomato skin.For the tomato skin powder,it can be extracted by hexane,acetone,ethyl acetate or dichloromethane.For the tomato skin pulp,it can remove the moisture use methanol or anhydrous ethanol,then extract with hexane,and test the eluent and extract solution at last,combing the result together.It can use any methods above to evaluate the lycopene content in tomato skin.