ASE-GC-MS/MS法测定海产品中苯并(a)芘含量方法的建立

ASE-GC-MS/MS Method for Research of Determination of Benzopyrene in Marine Products

建立快速溶剂萃取-气相色谱-三重四级杆串联质谱(GC-MS/MS)法测定海产品中的苯并(a)芘的分析方法。方法利用快速溶剂萃取仪进行提取,以丙酮和乙腈体系(2︰1, V/V)为提取溶剂, GC-MS/MS法进行定性定量分析。结果表明,苯并(a)芘浓度在0.5~20 ng/mL范围内线性关系良好,相关系数为0.999 1;检出限为0.03μg/kg;两种海产品加标回收率(低点0.1μg/kg,中点0.4μg/kg,高点4.0μg/kg)分别为94.9%和90.0%;精密度RSD (n=6)分别为1.39%和1.65%;重复性RSD (n=6)分别为3.83%和3.26%。试验证明,该方法具有前处理简单、分析速度快、检出限低、检测结果准确等优点。

An ASE-GC-MS/MS method for the determination of benzopyrene in marine products was established.In this method,rapid solvent extraction apparatus was used,acetone and acetontrile system(2︰1,V/V)was selected as extracted solution,and samples were tested qualitatively and quantitatively by GC-MS/MS.The result showed that benzopyrene had good linear relationships in the range of 0.5 to 20 ng/mL,with correlation coefficients of 0.991.Detection limits of benzopyrene were 0.03μg/kg.The recoveries in 2 kinds of marine products were 94.9%and 90.0%(Lower 0.1μg/kg,middle 0.4μg/kg and higher 4.0μg/kg),repectively.The RSD(n=6)of precision was 1.39%and 1.65%,and RSD(n=6)of reproducibility was 3.83%and 3.26%.It is proved that this method is simple pre-treated,rapid analysis,low detection and accuracy.