HPLC法测定禽蛋蛋黄中玉米黄质和叶黄素

Detection of Zeaxanthin and Lutein in Various Poultry Yolk by HPLC

建立高效液相色谱(HPLC)快速准确检测禽蛋蛋黄中玉米黄质和叶黄素的方法。色谱柱SHIMADZU?C18柱(4.6 mm×250 mm, 5μm),流动相为V乙腈P︰V甲醇︰V二氯甲烷=85︰10︰5,流速1 mL/min,检测波长450 nm,温度为室温。确定以甲醇、乙酸乙酯和正己烷为混合提取溶剂,经水洗、盐洗、碱洗和水洗,制备得到待测液。结果表明,玉米黄质和叶黄素分别在10~160μg/m L和15~240μg/m L内呈良好线性关系,相关系数分别为0.997和0.999;玉米黄质的平均加标回收率为97.8%, RSD为1.19%(N=6);叶黄素的平均加标回收率为98.2%, RSD为1.90%(N=6);玉米黄质和叶黄素的精密度(RSD)分别为1.44%和1.77%,检出限分别为0.094μg/m L和0.048μg/m L。建立的HPLC方法能快速准确检测草鸡蛋、饲料鸡蛋、茶叶鸡蛋、鹌鹑蛋、鸭蛋和鹅蛋中蛋黄的玉米黄质和叶黄素含量,其中草鸡蛋蛋黄中叶黄素和玉米黄质含量最高,分别为57.50 mg/kg和56.87 mg/kg。此次试验为评价禽蛋的营养价值提供重要参考。

A rapid and accurate method for the determination of zeaxanthin and lutein in various egg yolks by high performance liquid chromatography(HPLC)was established.Chromatographic condition was SHIMADZU?C18 column(4.6 mm×250 mm,5μm)with Vacetonitrile︰Vmethanol︰Vdichloromethane=85︰10︰5 as mobile phase,flow rate of 1 mL/min,detection wavelength of 450 nm and the room temperature.The mixture of methanol,ethyl acetate and n-hexane was used as extracted solution,and the extracting solutions of egg yolks were washed by water,salt,alkali and water,then used as tested solution.The zeaxanthin and lutein showed good linearities in the range of 10-160μg/mL and 15-240μg/mL,respectively.The correlation coefficients were 0.997 and 0.999,respectively.The average spiked recovery rate of zeaxanthin was 97.8%;RSD was 1.19%(N=6);The average spiked recovery rate of lutein was 98.2%;RSD was 1.90%(N=6).The precisions(RSD)of zeaxanthin and lutein were 1.44%and 1.77%,respectively,and the detection limits were 0.094μg/mL and 0.048μg/mL,respectively.The HPLC method could quickly and accurately separate and detect the content of zeaxanthin and lutein in the egg yolks of cottage eggs,feed eggs,tea eggs,quail eggs,duck eggs and goose eggs.The content of lutein and zeaxanthin in egg yolks of cottage eggs was highest at 57.50 mg/kg and 56.87 mg/kg,respectively,which provided an important reference for evaluating the nutritional value of eggs.