不同方法提取费约果叶片香气成分的比较研究

Comparison of aromatic components in feijoa sellowiana with different extraction methods

分别采用索氏抽提法、超声波辅助溶剂萃取法和顶空固相微萃取法对费约果叶片中所含香气成分进行提取,经GC-MS进行分析,结合谱库检索技术,采用峰面积归一化法进行定性和定量分析。结果表明,索氏抽提法所得香气物质中鉴定出挥发性成分20种,占总挥发性成分的70.357%,且以烷烃为主,超声波辅助溶剂萃取法所得香气物质中鉴定出挥发性成分17种,占总挥发性成分的65.222%,且以酯类为主,顶空固相微萃取法所得香气物质中鉴定出挥发性成分最多,有42种,占总挥发性成分的76.899%,且以萜烯类为主。由此可见,在费约果叶片香气成分分析中顶空固相微萃取法能得到最多的香气组分,索氏抽提法次之,超声波辅助溶剂萃取法得到的香气物质最少。

The aromatic components in fruit of Feijoa sellowiana were extracted by soxhlet extraction,ultrasonic assisted solvent extraction,and headspace solid phase micro- extraction,analyzed by gas chromatography- mass spectrometry,combined with the spectral library retrieval technology,and determined by peak area normalization method.The results showed that there were 20 compounds identified by soxhlet extraction and the percent of total peak area was 70.357% and the major compound was alkane,there were 17 compounds identified by ultrasonic assisted solvent extraction and the percent of total peak area was 65.222% and the major compounds was ester,and there were 42 compounds by headspace solid phase micro- extraction,76.899% of total peak area and the major compounds was terpenoid. This showed that the most kind of aroma compositions can be obtained by headspace solid phase micro- extraction,followed by soxhlet extraction,and the least aroma compositions can be obtained by ultrasonic assisted solvent extraction.