滇橄榄对人口腔唾液流率、pH及细菌的影响研究

Effect of the Fructus phyllanthi on the human saliva flow rate,pH and bacterial

目的:研究滇橄榄对人体口腔唾液流率、pH及口腔细菌的影响。方法:分时段采集10名志愿者咀嚼滇橄榄前、咀嚼滇橄榄期间、咀嚼滇橄榄后的唾液,检测志愿者唾液流率及pH的变化,同时采用荧光定量PCR实验法检测唾液中的总细菌及变形链球菌含量。结果:与咀嚼前相比,咀嚼滇橄榄后志愿者分泌唾液的量显著增加,唾液的p H先降低后升高,口腔内细菌及变形链球菌的数目有所减少。结论:滇橄榄有促进人体口腔唾液分泌的作用,可以有效减少口腔内的细菌总数及变形链球菌数量,有一定预防龋齿的效果。

Objective: To study the effect of the Fructus phyllanthi on the human saliva flow rate,p H and bacterial.Methods: The saliva both before and after chewing form 10 volunteers were collected,the saliva flow rate and pH change were determined and the total bacteria and Streptococcus mutans content in saliva were detected using real- time PCR experiment. Results: Compared with the results before chewing,volunteers saliva flow rate was increased significantly after chewing Fructus phyllanthi,the pH was decreased first and then increased,the number of total bacteria and S. mutans were decreased. Conclusion: Fructus phyllanthi could promote human saliva secretion,and effectively reduce the number of total bacteria and S.mutans in the oral,there was a certain effect on the prevention of dental caries.