市售鲜肉中奇异变形杆菌的ERIC-PCR分型与耐药性分析

Analysis of ERIC-PCR Typing and Drug Resistance of Proteus mirabilis in Commercially Available Fresh Meat

目的:研究中山市市售生鲜肉中分离的奇异变形杆菌的同源性和耐药性,分析其基因型与耐药表型之间的关系。方法:采用肠杆菌基因间重复序列(Entrobacter repetive intergenic consensus,ERIC)-PCR分型方法对71株奇异变形杆菌进行分型,所得分型指纹图谱使用Quantity One软件进行处理分析。耐药性检测采用K-B纸片扩散法,参照CLSI制定的标准判读药敏结果。结果:71株奇异变形杆菌的相似性系数为0.64～1.00,以75%为界可分为9个簇。测试的18种抗生素中,对链霉素和红霉素的耐药率最高,均为100%;对青霉素和头孢曲松耐药率最低,分别为16.90%、11.27%。所有菌株均为多重耐药菌,且至少耐6种药物。结论:市售生鲜肉中分离的71株奇异变形杆菌相似性高,耐药情况严重,其基因型与耐药表型之间没有明显的相关性。

Objective: To investigate the homology and drug resistance of Proteus mirabilis strains isolated from commercially available fresh meat in Zhongshan city and analyze the relationship between their genotypes and drug resistance phenotypes.Methods: 71 P. mirabilis strains were classified by the enterobacterial repetitive intergenic consensus( ERIC)-PCR typing method and the acquired fingerprints were processed with Quantity One software. The Kirby-Bauer disk diffusion method was used for the antimicrobial resistance susceptibility testing,and the results were interpreted according to the standards established by the CLSI.Results: The similarity coefficients of 71 P.mirabilis strains were 0.64~1.00,and they were divided into9 clusters bounded by 75%. In the 18 antibiotics tested,the strains had the highest rates of resistance to streptomycin and erythromycin,both reached 100%. The resistance rates to penicillin and ceftriaxone were the lowest,16.90% and 11.27%,respectively.All strains were of multidrug resistance and were resistant to 6 drugs at least. Conclusion: 71 P. mirabilis strains isolated from commercial available fresh meat had high similarity and serious drug resistance.There was no obvious correlation between their genotypes and resistance phenotypes.