响应面优化微波辅助提取蓝靛果花色苷工艺及其抗氧化活性

Optimization of Microwave-assisted Extraction of Anthocyanins from Lonicera edulis and Its Antioxidant Activity by Response Surface Methodology

为了充分利用野生蓝靛果,提高其经济价值,研究蓝靛果花色苷的微波辅助提取工艺及其体外抗氧化活性。本文以小兴安岭野生蓝靛果为实验原材料,应用响应面法优化提取蓝靛果花色苷工艺,并分析了其对3种自由基的清除能力及其总还原力。结果表明,微波辅助提取蓝靛果花色苷的最优工艺条件为微波功率280 W、微波时间90 s、料液比1∶25 g/mL、乙醇体积分数85%,在此工艺条件下,蓝靛果花色苷的提取量达(292.16±1.25) mg/100 g。蓝靛果花色苷有一定的抗氧化能力,对DPPH自由基、ABTS^+·和超氧阴离子自由基有较高的清除能力,清除率分别达到89.20%、70.40%和44.73%,同时有较高的总还原能力。该研究为从蓝靛果中提取花色苷提供了一种经济可行的方法,进一步挖掘了蓝靛果的价值。

In order to make full use of wild Lonicera edulis and improve its value,the microwave-assisted extraction of anthocyanin from Lonicera edulis and its antioxidant activity in vitro were studied.In this paper,the wild Lonicera edulis fruit in Xiaoxingan Mountains was used as the experimental raw material.Optimization of anthocyanin extraction from Lonicera edulis by Response Surface Methodology,the scavenging ability to 3 kinds of free radicals and total reducing power were also analyzed.The results showed that microwave assisted extraction of anthocyanins from Lonicera edulis anthocyanins was microwave power280 W,microwave time 90 s,material liquid ratio 1 ∶ 25 g/mL,and ethanol volume fraction 85%. Under the conditions,the extraction of anthocyanin of Lonicera edulis was( 292.16 ± 1.25) mg/100 g.The anthocyanins of Lonicera edulis have a certain antioxidant capacity,and have high scavenging ability for DPPH free radical,ABTS^+· and superoxide anion radical. The scavenging rate was 89.2%,70.4% and 44.73% respectively,and the total reduction ability was higher.This study provided an economical and feasible method for extracting anthocyanins from Lonicera edulis and further explored the value of Lonicera edulis.