复合酶法同步提取葡萄籽粕蛋白质和可溶性膳食纤维的响应面优化

Response Surface Optimization of Simultaneous Extraction of Protein and Soluble Dietary Fiber from Grape Seed Meal by Complex Enzymatic Method

用复合酶法同步提取葡萄籽粕蛋白质和可溶性膳食纤维。实验选用木瓜蛋白酶和纤维素酶进行提取,在单因素实验的基础上,应用响应面法优化提取条件,确定复合酶提取葡萄籽粕蛋白质和可溶性膳食纤维的提取率。结果表明,优化工艺为:木瓜蛋白酶和纤维素酶之比3∶1,提取温度50℃,料液比1∶20 (g/m L),提取pH7.0,提取时间50 min,蛋白质的提取率为83.07%±1.43%、可溶性膳食纤维得率为34.04%±0.87%。因此,选用复合酶法对葡萄籽粕中蛋白质和可溶性膳食纤维进行同步提取具有一定的可行性,也为其他副产物同步提取蛋白质与可溶性膳食纤维提供了理论指导。

The protein and soluble dietary fiber of grape seed meal were extracted by complex enzymatic method.Papain and cellulase were used in the experiment to extract. On the basis of single factor experiments,response surface methodology was applied to optimize the extraction conditions.The extraction rate of protein and soluble dietary fiber from grape seed meal was determined by the complex enzyme.The optimum conditions were as follows: the papain and cellulase was added 3 ∶1,the extraction temperature was 50 ℃,the ratio of material to liquid was 1∶20(g/m L),p H7.0,the extraction time was 50 min.The extraction rate of protein was 83.07% ± 1.43% and the extraction rate of soluble dietary fiber was 34.04% ±0.87%.Therefore,it was feasible to select the synchronous extraction of protein and soluble dietary fiber from grape seed meal by using complex enzymatic method. It also provided theoretical guidance for the synchronous extraction of protein and soluble dietary fiber by other by-products.